青云英语翻译
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a为了确定已克隆的辣椒多聚半乳糖醛酸酶基因(CaPG)在辣椒耐贮性等方面所起的作用,本试验拟在获得该基因全长cDNA序列的基础上，构建该基因由组成型启动子CaMV35S超量表达载体，为植物材料遗传转化的方法鉴定该基因的功能做准备。方法：将CaPG全长基因的PCR产物回收后，按一定比例与克隆载体连接，将连接产物转入到大肠杆菌DH5α，培养检测。将含有目的基因的重组质粒经双酶切，同时将表达载体用相同的限制性内切酶进行酶切，然后利用T4DNA连接酶连接，得到重组质粒。转化大肠杆菌DH5α，筛选重组子。提取阳性克隆质粒，经PCR方法检测得到重组克隆载体。 In order to determine already clones because the hot pepper gathers the galacturonic acid substrat (CaPG) the role which in aspects and so on hot pepper keeping quality plays, this experiment draws up in obtains this gene span cDNA sequence in the foundation, constructs this gene by to be composed t [translate]
ahope you can understand [translate]
a数字电子技术 Numeral electronic technology [translate]
a2016年 In 2016 [translate]
aHelp... is the last option in each of 正在翻译，请等待... [translate]
aare just like a double-edged sword. With them we may have less trouble dealing with problems in life and enjoy a better-off life. However, one point should be kept in mind that we should take sensible use of them , always being the master of them. 是象一把双刃剑。 与他们我们在生活中也许有较少麻烦应付问题和享有better-off生活。 然而的一点我们应该采取易察觉的用途对他们应该记住，总是大师他们。 [translate]
acorrection by the author(s). The material, as presamed, does not necessarily ?eflact any [translate]
ais longlong very youne Do you love me a littl ending... 是longlong非常youne您爱我littl结尾… [translate]
aThe salesman showed his customer to the door, and the young man left the shop without a word. He went to a showroom on the other side of the street and asked for sixteen cars. The second salesman was polite and helpful. The young man took a bundle of bank notes out of his pocket and paid for the cars in cash. He explai [translate]
atumor burden 肿瘤负担 [translate]
ai made steak for my daughter this morning 我今晨做了牛排为我的女儿 [translate]
a气路管 Air course tube [translate]
a产品生产分布统计 Product production distribution statistics [translate]
a请按附件中规定的时间送仓，谢谢！ Please the time which stipulated according to the appendix in do deliver the warehouse, thanks! [translate]
aplain-spoken 言辞坦率 [translate]
aLAN addresses,ARP LAN地址， ARP [translate]
a整个教学内容主要围绕“会做什么”这个话题展开 The entire course content main encompassment “can make any” this topic to launch [translate]
aWrite a short passage about yourself 正在翻译，请等待... [translate]
aMaybe I still care about youMaybe I still care about youMaybe I still care about you 可能我对youMaybe I寂静的关心仍然关心关于youMaybe I寂静的关心关于您 [translate]
a难啊难 Difficult difficult [translate]
a申请加入费 Applies to join the expense [translate]
aWireless links characteristics 无线电线路特征 [translate]
aI have informed Landy Chen already. 我已经通知了Landy陈。 [translate]
aThis policy of Insurance witnesses that the People’s Insurance Company of China(hereinafter called “The Company”) 保险证人这项政策中国的People的Insurance Company (以后称“公司”) [translate]
a从沟通能力来说，不能与各部门同事有效沟通 From the communication ability, cannot communicate effectively with various departments colleague [translate]
aA budget starts taking its own life after it is approved A budget starts taking its own life after it is approved [translate]
a不要在吃饭的时候忙别的事情BE BUSY DOING Does not want time busy other matter BE BUSY DOING which eats meal [translate]
a不是你懒的换,是你现在已经换不掉了 正在翻译，请等待... [translate]
a为了确定已克隆的辣椒多聚半乳糖醛酸酶基因(CaPG)在辣椒耐贮性等方面所起的作用,本试验拟在获得该基因全长cDNA序列的基础上，构建该基因由组成型启动子CaMV35S超量表达载体，为植物材料遗传转化的方法鉴定该基因的功能做准备。方法：将CaPG全长基因的PCR产物回收后，按一定比例与克隆载体连接，将连接产物转入到大肠杆菌DH5α，培养检测。将含有目的基因的重组质粒经双酶切，同时将表达载体用相同的限制性内切酶进行酶切，然后利用T4DNA连接酶连接，得到重组质粒。转化大肠杆菌DH5α，筛选重组子。提取阳性克隆质粒，经PCR方法检测得到重组克隆载体。 In order to determine already clones because the hot pepper gathers the galacturonic acid substrat (CaPG) the role which in aspects and so on hot pepper keeping quality plays, this experiment draws up in obtains this gene span cDNA sequence in the foundation, constructs this gene by to be composed t [translate]
ahope you can understand [translate]
a数字电子技术 Numeral electronic technology [translate]
a2016年 In 2016 [translate]
aHelp... is the last option in each of 正在翻译，请等待... [translate]
aare just like a double-edged sword. With them we may have less trouble dealing with problems in life and enjoy a better-off life. However, one point should be kept in mind that we should take sensible use of them , always being the master of them. 是象一把双刃剑。 与他们我们在生活中也许有较少麻烦应付问题和享有better-off生活。 然而的一点我们应该采取易察觉的用途对他们应该记住，总是大师他们。 [translate]
acorrection by the author(s). The material, as presamed, does not necessarily ?eflact any [translate]
ais longlong very youne Do you love me a littl ending... 是longlong非常youne您爱我littl结尾… [translate]
aThe salesman showed his customer to the door, and the young man left the shop without a word. He went to a showroom on the other side of the street and asked for sixteen cars. The second salesman was polite and helpful. The young man took a bundle of bank notes out of his pocket and paid for the cars in cash. He explai [translate]
atumor burden 肿瘤负担 [translate]
ai made steak for my daughter this morning 我今晨做了牛排为我的女儿 [translate]
a气路管 Air course tube [translate]
a产品生产分布统计 Product production distribution statistics [translate]
a请按附件中规定的时间送仓，谢谢！ Please the time which stipulated according to the appendix in do deliver the warehouse, thanks! [translate]
aplain-spoken 言辞坦率 [translate]
aLAN addresses,ARP LAN地址， ARP [translate]
a整个教学内容主要围绕“会做什么”这个话题展开 The entire course content main encompassment “can make any” this topic to launch [translate]
aWrite a short passage about yourself 正在翻译，请等待... [translate]
aMaybe I still care about youMaybe I still care about youMaybe I still care about you 可能我对youMaybe I寂静的关心仍然关心关于youMaybe I寂静的关心关于您 [translate]
a难啊难 Difficult difficult [translate]
a申请加入费 Applies to join the expense [translate]
aWireless links characteristics 无线电线路特征 [translate]
aI have informed Landy Chen already. 我已经通知了Landy陈。 [translate]
aThis policy of Insurance witnesses that the People’s Insurance Company of China(hereinafter called “The Company”) 保险证人这项政策中国的People的Insurance Company (以后称“公司”) [translate]
a从沟通能力来说，不能与各部门同事有效沟通 From the communication ability, cannot communicate effectively with various departments colleague [translate]
aA budget starts taking its own life after it is approved A budget starts taking its own life after it is approved [translate]
a不要在吃饭的时候忙别的事情BE BUSY DOING Does not want time busy other matter BE BUSY DOING which eats meal [translate]
a不是你懒的换,是你现在已经换不掉了 正在翻译，请等待... [translate]
